SLC9A3 antibody (Intracellular)

Catalog No. ABIN7043758

Product Details anti-SLC9A3 Antibody

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Binding Specificity: AA 466-479, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Rat

Predicted Reactivity: 14 amino acid residues identical,Mouse - 13, human - 12

Characteristics: Anti-Na+/H+ Exchanger 3 Antibody is directed against an epitope of rat . Anti-Na+/H+ Exchanger 3 Antibody (ABIN7043758, ABIN7044881 and ABIN7044882) can be used in western blot analysis. It has been designed to recognize from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237211)

Blocking Peptide: (ABIN7237211)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC9A3

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Alternative Name: SLC9A3 (SLC9A3 Products)

Background: Sodium/hydrogen exchanger 3, SLC9A3,Maintaining physiological intracellular pH values is vital for most cells. The Na+/H+ exchanger family (NHE, also known as SLC9) is widely expressed and has a key role in protecting cells against acidification. There are currently six NHE transporters identified in mammalian cells1. The complete structure of members of the NHE family has not been completely solved. However, all members are organized in a similar manner with 11 or 12 membrane spanning domains, carrying out the Na+ and H+ exchange and a varying length intracellular C-terminal domain being involved in regulation of the exchange activity. The transport of Na+ and H+ in NHEs occurs in the center of the N-terminus via a two inverted funnel area that is made up of two transmembrane regions. NHE3 is expressed in sodium absorptive cells of the mammalian small intestine, colon, gall bladder, renal proximal tubule, and thick and thin limbs of the loop of Henle and can be mostly found in the plasma membrane2. NHE3 interacts with a multi-PDZ domain-containing scaffold protein called NHERF-1. NHERF-1 binds to the NHE3 C-terminus and has a role in NHE3 regulation in various cells. NHERF-1 functions as a cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP3. In addition, silencing of NHERF-1 in a model of diabetic mice was found to prevent NHE3 trafficking and insulin-dependent activation4.

Alternative names: Na+/H+ Exchanger 3 (NHE-3), Sodium/hydrogen exchanger 3, SLC9A3

Gene ID: 24784

NCBI Accession: NM_004174

UniProt: P26433

Pathways: Proton Transport