SLC9A6 antibody (Intracellular)

Catalog No. ABIN7043760

Product Details anti-SLC9A6 Antibody

Target: SLC9A6 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 6 (SLC9A6))

Binding Specificity: AA 515-528, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 6 (NHE-6)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 13,14 amino acid residues identical

Characteristics: Anti-Na+/H+ Exchanger 6 Antibody (ABIN7043760, ABIN7044871 and ABIN7044872) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHLGVPENERRTTK, corresponding to amino acid residues 515-528 of human NHE-6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237219)

Blocking Peptide: (ABIN7237219)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC9A6

Target: SLC9A6 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 6 (SLC9A6))

Alternative Name: SLC9A6 (SLC9A6 Products)

Background: Sodium/hydrogen exchanger 6, NHE6, SLC9A6,Intracellular pH regulation in the brain is extremely important in both physiological and pathophysiological conditions. Changes in Intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located on the plasma membrane called sodium-hydrogen exchangers (Na+/H+ exchanger, NHE)1,2. In addition to their role in regulating pH , NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume1.Several studies have suggested that Na+/H+ exchange is the predominant pH -regulatory mode in isolated nerve terminals. In addition, Na+/H+ exchange plays a role in regulating synaptic transmission at glutamatergic, GABAergic, and dopaminergic synapses1.The NHE family belongs to the Slc9 gene family of Na+ coupled transport proteins and comprises nine mammalian isoforms of Na+/H+ exchangers that are localized to the plasma membrane, NHE1-9 encoded by the SLC9A1-SLC9A9 genes, respectively. SLC9 family members have a short N terminus and an extensive C terminus that plays a regulatory role2.NHE6 is localized to early and recycling endosomes, where it exchanges luminal H+ for Na+ or K+. Its expression is highest in the spinal cord and brain and is particularly enriched in the hippocampus, cortex, and Purkinje cell layer of the cerebellum3. Mutations in NHE6 encoding gene have been linked to autism, Christianson syndrome, and Angelman-like syndrome.

Alternative names: Na+/H+ Exchanger 6 (NHE-6), Sodium/hydrogen exchanger 6, NHE6, SLC9A6

Gene ID: 10479

NCBI Accession: NM_006359

UniProt: Q92581