S1PR2 antibody (AA 129-146)

Catalog No. ABIN7043773

Product Details anti-S1PR2 Antibody

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Binding Specificity: AA 129-146

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2

Specificity: 2nd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 17,18 amino acid residues identical, human - 16

Characteristics: Anti-S1PR2 (EDG5) Antibody is directed against an intracellular epitope of mouse sphingosine 1-phosphate receptor 2. Anti-S1PR2 (EDG5) Antibody (ABIN7043773, ABIN7045272 and ABIN7045273)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize S1PR2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-SR012

Blocking Peptide: BLP-SR012

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for S1PR2

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Alternative Name: S1PR2 (S1PR2 Products)

Background: Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2,Lysophospholipids constitute a group of important lipid mediators, lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG), S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.

Alternative names: S1PR2 (EDG5), Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2

Gene ID: 14739

NCBI Accession: NM_004230

UniProt: P52592

Pathways: Synaptic Membrane