SYNDIG1 antibody (Intracellular)

Catalog No. ABIN7043785

Product Details anti-SYNDIG1 Antibody

Target: SYNDIG1 (Synapse Differentiation Inducing 1 (SYNDIG1))

Binding Specificity: AA 119-132, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYNDIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to SynDIG1

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-SynDIG1 Antibody (ABIN7043785, ABIN7044392 and ABIN7044393)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SynDIG1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSPTKDSLEYPDGK, corresponding to amino acid residues 119-132 of rat SynDIG1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-GC049

Blocking Peptide: BLP-GC049

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SYNDIG1

Target: SYNDIG1 (Synapse Differentiation Inducing 1 (SYNDIG1))

Alternative Name: SYNDIG1 (SYNDIG1 Products)

Background: Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin subfamily C member 2, DSPC2,SynDIG1 (Synapse differentiation induced gene 1) is a brain-specific, highly conserved type II integral membrane protein detected at excitatory synapses. The protein is responsible for the regulation of excitatory synapse number and strength in hippocampal neurons. The protein plays an important role during synapse development in regulating AMPA receptor (AMPAR) and PSD-95 content at excitatory synapses1,2.SynDIG family includes four members: SynDIG1-4. SynDIG1 structure includes a large intracellular N-terminal region followed by a single transmembrane domain and a second hydrophobic segment that does not span the membrane. The membrane-associated region forms a three-helical bundle with two cysteine residues located at positions 191 and 192 in the juxta-transmembrane region exposed to the cytoplasm1,2.In studies conducted on mice, deletion of endogenous SynDIG1 in the mouse brain results in a significant decrease in excitatory synapse maturation, but not in synapse composition. Thus, SynDIG1 is not required for synaptogenesis in vivo1. Contrarily, overexpression of SynDIG1 in cultures of dissociated rat hippocampal neurons shows a significant increase in excitatory synapse strength and number.

Alternative names: SynDIG1, Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin subfamily C member 2, DSPC2

Gene ID: 362235

NCBI Accession: NM_024893

UniProt: Q58DZ9

Pathways: Synaptic Membrane