TRPC4 antibody (Extracellular)

Catalog No. ABIN7043822

Product Details anti-TRPC4 Antibody

Target: TRPC4 (Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4))

Binding Specificity: AA 458-469, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to TRPC4 Channel

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-TRPC4 (extracellular) Antibody (ABIN7043822, ABIN7044067 and ABIN7044068) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPC4 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|ICC

Negative Control: (ABIN7237424)

Blocking Peptide: (ABIN7237424)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPC4

Target: TRPC4 (Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4))

Alternative Name: TRPC4 (TRPC4 Products)

Background: Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1,The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1) TRPC1 2) TRPC4 and TRPC5 3) TRPC3, TRPC6, TRPC7 4) TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by Ca2+ store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6.

Alternative names: TRPC4, Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1

Gene ID: 84494

NCBI Accession: NM_003306

UniProt: O35119