TRPM1 antibody (Intracellular)

Catalog No. ABIN7043828

Product Details anti-TRPM1 Antibody

Target: TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1 (TRPM1))

Binding Specificity: AA 1518-1533, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Human TRPM1 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity (Details): Will not recognize rat or mouse TRPM1.

Predicted Reactivity: Canis - 12,16 amino acid residues identical

Characteristics: Anti-Human TRPM1 Antibody (ABIN7043828, ABIN7044004 and ABIN7044005) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPM1 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EEKKVKKEKASTETE, corresponding to amino acid residues 1518-1533 of human TRPM1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPM1

Target: TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1 (TRPM1))

Alternative Name: TRPM1 (TRPM1 Products)

Background: Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1,The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas. The TRPM family consist of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3, TRPM2 and TRPM8, TRPM4 and TRPM5, and TRPM6 and TRPM7.The TRPM proteins share structural homology with other members of the TRP superfamily channels, six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family. TRPM channels were suggested to play a role in tumourigenesis, proliferation and differentiation.Recently, TRPM1 was found to be down-regulated in a highly metastatic line. The loss of TRPM1 expression has been reported to be correlated to melanoma aggressiveness.Two alternative splice variants of TRPM1 were reported, short (TRPM1-S) and long (TRPM1-L). The TRPM1-S was described to be expressed at human melanocyte and mainly in metastasic melanoma cell lines. While TRPM1-L is expressed at murine CNS.

Alternative names: TRPM1, Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1

Gene ID: 4308

NCBI Accession: NM_002420

UniProt: O75560