TRPM3 antibody (Extracellular)

Catalog No. ABIN7043832

Product Details anti-TRPM3 Antibody

Target: TRPM3 (Transient Receptor Potential Cation Channel, Subfamily M, Member 3 (TRPM3))

Binding Specificity: AA 816-831, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRPM3 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 15,16 amino acid residues identical

Characteristics: Anti-TRPM3 (extracellular) Antibody (ABIN7043832, ABIN7044018 and ABIN7044019) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237450)

Blocking Peptide: (ABIN7237450)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPM3

Target: TRPM3 (Transient Receptor Potential Cation Channel, Subfamily M, Member 3 (TRPM3))

Alternative Name: TRPM3 (TRPM3 Products)

Background: Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2,Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH , endocrine factors as well as signaling molecules2. The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 - a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.The TRPM subfamily consists of 8 members divided in to three major groups: TRPM1/3, TRPM4/5, and TRPM6/7. TRPM2 and TRPM8 are not included in any of the groups since they are quite different even with respect to each other. TRPM3 is alternatively spliced into active variants5,6. TRPM3a1 and TRPM3a2 in mouse are monovalent and divalent-selective channels, respectively. This differential selectivity strongly suggests that their cellular role depends on the relative expression of each variant type. In addition, both splice variants display constitutive outwardly rectifying currents. They somewhat resemble TRPM6 and TRPM7 channels such that their currents are inhibited by intracellular Mg2+ 1. TRPM3 is highly expressed in the kidney, and to a lesser extent in the brain, testis, and spinal cord7.

Alternative names: TRPM3, Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2

Gene ID: 80036

NCBI Accession: NM_206945

UniProt: Q9HCF6