TRPV1 antibody (Intracellular)

Catalog No. ABIN7043841

Product Details anti-TRPV1 Antibody

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Binding Specificity: AA 824-838, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRPV1 (VR1) Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,guinea pig - 14,15 amino acid residues identical, human - 10

Characteristics: Anti-TRPV1 (VR1) Antibody (ABIN7043841, ABIN7043988 and ABIN7043989) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|Whole Tissue Staining

Negative Control: (ABIN7237478)

Blocking Peptide: (ABIN7237478)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPV1

Target: TRPV1 (Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1))

Alternative Name: TRPV1 (TRPV1 Products)

Background: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1,TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.5,6Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Alternative names: TRPV1 (VR1), Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Gene ID: 83810

NCBI Accession: NM_018727

UniProt: O35433

Pathways: Dicarboxylic Acid Transport