TMC6 antibody (Intracellular)

Catalog No. ABIN7043858

Product Details anti-TMC6 Antibody

Target: TMC6 (Transmembrane Channel-Like 6 (TMC6))

Binding Specificity: AA 681-695, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMC6 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to TMC6 Channel

Specificity: Intracellular, cytoplasm

Cross-Reactivity: Human, Rat

Predicted Reactivity: Rat,mouse - 13,14 amino acid residues are identical

Characteristics: Anti-TMC6 (EVER1) Antibody is directed against an epitope of the human Transmembrane channel-like protein 6. Anti-TMC6 (EVER1) Antibody (ABIN7043858, ABIN7045316 and ABIN7045317) can be used in western blot analysis. It has been designed to TMC6 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CGPFRTLDTMYEAGR, corresponding to amino acid residues 681-695 of human TMC6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:800

Comment:

Negative Control: (ABIN7237513)

Blocking Peptide: (ABIN7237513)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMC6

Target: TMC6 (Transmembrane Channel-Like 6 (TMC6))

Alternative Name: TMC6 (TMC6 Products)

Background: Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, Protein LAK-4, LAK4P, EVIN1, EV1,The EVER gene belongs to a larger family of proteins referred to as the transmembrane channel-like protein (TMC) family1. The EVER1/TMC6 integral membrane protein, is located in the endoplasmic reticulum (ER) of keratinocytes, where it forms a complex with zinc transporter-1 (ZnT-1), a membrane protein responsible for zinc efflux and resistance to zinc toxicity, and thereby controlling the cellular zinc balance2. The EVER1 protein is predicted to have 10 transmembrane domains and 2 leucine zipper motifs1.The EVER1 gene encodes 2 alternatively spliced proteins, one 805 amino acids long and the other 454 amino acids, in the same open reading frame. The EVER genes are thought to be transcribed in numerous tissues including the skin1. Mutations in the TMC6 are implicated in the development of the rare autosomal recessive disease Epidermodysplasia Verruciformis (EV)3. EV is characterized by extreme susceptibility to infection by cutaneous human papillomaviruses (HPV) of the beta genus. The TMC6 genes may be involved in controlling HPV gene expression and replication in epidermal keratinocytes or they may directly affect innate and adaptive immune responses which control the clearance of HPV-infected keratinocytes in EV cases4. A recent report indicated an association between genetic variation in the TMC6 region and development of cervical cancer (CxCa)5.

Alternative names: TMC6 (EVER1), Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, Protein LAK-4, LAK4P, EVIN1, EV1

Gene ID: 11322

NCBI Accession: NM_001127198

UniProt: Q7Z403