Recombinant TIMD2 antibody (Extracellular Domain)

Catalog No. ABIN7072511

Product Details anti-TIMD2 Antibody

Target: TIMD2 (T Cell Immunoglobulin and Mucin Domain Containing 2 (TIMD2))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 1-230, Extracellular Domain

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This TIMD2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Blocking Peptide (BP), Immunohistochemistry (IHC), Functional Studies (Func)

Purpose: Anti-TIM-2 [RMT2-14], Rabbit IgG, lambda

Specificity: This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.

Clone: RMT2-14

Isotype: IgG lambda

Application Details

Application Notes: This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TIMD2

Target: TIMD2 (T Cell Immunoglobulin and Mucin Domain Containing 2 (TIMD2))

Alternative Name: TIM-2 (TIMD2 Products)

Background: TIMD2, T Cell Immunoglobulin Mucin-2, T Cell immunoglobulin and mucin domain protein 2, TIM3

UniProt: Q8R183