Recombinant VISTA antibody

Catalog No. ABIN7072594

Product Details anti-VISTA Antibody

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This VISTA antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Blocking Peptide (BP)

Purpose: Anti-VISTA [13F3], Rabbit IgG, lambda

Specificity: This antibody is specific for murine VISTA.

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein.

Clone: 13F3

Isotype: IgG lambda

Application Details

Application Notes: The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017, Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for VISTA

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: VISTA (VISTA Products)

Background: PD-1H, V-domain Ig suppressor of T cell activation, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, Vsir, Dies1

UniProt: Q9D659

Pathways: Cancer Immune Checkpoints