Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody

Details for Product No. ABIN708086
Antigen
  • CD142
  • TF
  • TFA
  • f3
  • AA409063
  • Cf-3
  • Cf3
  • tf
  • zgc:112151
  • coagulation factor III, tissue factor
  • coagulation factor IIIa
  • coagulation factor III
  • tissue factor
  • coagulation factor IIIb
  • coagulation factor III (thromboplastin, tissue factor) S homeolog
  • F3
  • f3a
  • tf
  • f3b
  • f3.S
Epitope
AA 40-90
15
15
12
11
9
9
8
6
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
180
50
44
20
17
3
3
1
Host
Rabbit
141
72
16
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
27
25
7
5
4
3
3
3
3
3
3
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
153
84
68
62
21
19
16
12
12
11
9
4
4
3
3
2
2
1
1
1
1
Options
'Independent Validation' Badge
Lot Number 980886W
Method validated Immunohistochemistry
Positive Control Mouse lung tissue
Negative Control Mouse brain tissue
Notes Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.
Primary Antibody
  • Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
  • Catalog number: ABIN708086
  • Lot number: 120224
Isotype Control Antibody
  • Antibody: Rabbit IgG isotype control
  • Lot number: SLBD3695V
Secondary Antibody
  • Antibody: Bond Polymer Refine Detection Kit
  • Lot number: 24144
Controls
  • Positive control: Wild type mouse lung (specimen known to contain the target protein) from Explora BioLabs.
  • Negative Control: Wild type mouse brain (specimen known to not contain the target protein) from Explora BioLabs.
  • Primary antibody isotype control: Wild type mouse lung treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse lung treated with secondary antibody only (no primary antibody).
Protocol
  • Immunohistochemistry was performed on a Leica Bond automated immunostainer.
  • Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
  • Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
  • Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
  • Sections were washed x 3 in Leica Bond Wash Buffer.
  • Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
  • Sections were washed x 3 in Leica Bond Wash Buffer.
  • Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
  • Sections were washed x 4 in Leica Bond Wash Buffer.
  • Sections were washed x 1 in Distilled Water.
  • Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
  • Sections were washed x 4 in Leica Bond Wash Buffer.
  • Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
  • Sections were washed x 3 in Distilled Water.
  • Sections were counterstained with hematoxylin (Leica) for 2 minutes.
  • Sections were washed x 1 in Distilled Water.
  • Sections were washed x 1 in Leica Bond Wash Buffer.
  • Sections were washed x 1 in Distilled Water.
  • Sections were dehydrated, mounted and photographed under a light microscope.
Experimental Notes None notes.
Validation Images
Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Figure 1: Mouse lung tissue stained with anti-F3 (brown) and counterstained in blue.
Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Figure 2: Mouse brain tissue stained with anti-F3 (brown) and counterstained in blue.
Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Figure 3: Mouse lung tissue stained with isotype control antibody (brown) and counter...
Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Figure 4: Mouse lung tissue stained with secondary antibody only (brown) and counters...
Immunogen KLH conjugated synthetic peptide derived from human CD142
Isotype IgG
Purification Purified by Protein A.
Antigen
Alternative Name CD142 (F3 Antibody Abstract)
Background

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Subcellular location: Extracellular

Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

Gene ID 2152
UniProt P13726
Pathways Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD1...
Immunohistochemistry (IHC) image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Formalin-fixed and paraffin embedded rat lung labeled with Anti Tissue factor/CD142/F...
Immunohistochemistry (IHC) image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Independently Validated Antibody, image provided by Science Direct, badge number 0295...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD1...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Formalin-fixed and paraffin embedded rat lung labeled with Anti Tissue factor/CD142/F...
Western Blotting (WB) image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 40-90) antibody (ABIN708086) Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti Tissue factor/CD...
Product cited in: Fletcher, Das, Gadye, Street, Baudhuin, Wagner, Cole, Flores, Choi, Yosef, Purdom, Dudoit, Risso, Ngai: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, 2017 (PubMed). Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, 2016 (PubMed).

Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, 2016 (PubMed).

Gleeson, Martin, Ali, Kumar, Pillai, Kumar, OSullivan, Whelan, Stocca, Khider, Barry, OBrien, Caplice: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), 2015 (PubMed). (Sample species: Pig (Porcine)).

Tian, Salsbery, Wang, Yuan, Yang, Zhao, Wu, Zhang, Konkle, Thiagarajan, Li, Zhang, Dong: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, 2015 (PubMed).

Milano, Dongiovanni, Artoni, Gatti, Rosso, Colombo, Bollati, Maggioni, Mannucci, Bertazzi, Fargion, Valenti: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Liu, Wei, Zhang, Xu, Wang, Zhou: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, 2014 (PubMed).

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