CREB5 antibody

Catalog No. ABIN7127208

Product Details anti-CREB5 Antibody

Target: CREB5 (CAMP Responsive Element Binding Protein 5 (CREB5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CREB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.

Cross-Reactivity (Details): Human. Predicted to work with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CREB5 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or K562 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CREB5

Target: CREB5 (CAMP Responsive Element Binding Protein 5 (CREB5))

Alternative Name: CREB5 (CREB5 Products)

Background: CAMP response element-binding protein CREBPa (CRE-BPa), cAMP responsive element binding protein 5 isoform alpha, CRE BPA, CREBPA, cyclic AMP responsive element binding protein 5,CREB5 / CRE-BPa
Cellular localisation: Nucleus.

Molecular Weight: 57kDa

Gene ID: 9586, 437075

UniProt: Q02930

Pathways: Thyroid Hormone Synthesis