NFKBIE antibody (pSer22)

Catalog No. ABIN7138486

Product Details anti-NFKBIE Antibody

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Binding Specificity: pSer22

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB-e.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NFKBIE

Target: NFKBIE (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE))

Alternative Name: NFKBIE (NFKBIE Products)

Background:

Background: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Shirane M, et al. (1999) J Biol Chem, 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ,18, 621-663.
Chen, Z.J. et al. (1996) Cell, 84, 853-862.
Brown, K. et al. (1995) Science, 267, 1485-1488.

Aliases: I kappa B epsilon antibody, I-kappa-B-epsilon antibody, IkappaBepsilon antibody, IkB E antibody, IkB-E antibody, IkB-epsilon antibody, IKBE antibody, IKBE_HUMAN antibody, MGC72568 antibody, NF kappa B inhibitor epsilon antibody, NF kappa BIE antibody, NF-kappa-B inhibitor epsilon antibody, NF-kappa-BIE antibody, NFkappa BIE antibody, NFkappaB inhibitor epsilon antibody, NFKBIE antibody, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody, OTTHUMP00000016522 antibody, Slc35b2 antibody, solute carrier family 35, member B2 antibody

UniProt: O00221

Pathways: NF-kappaB Signaling, Maintenance of Protein Location