GPR139 antibody (Intracellular)

Catalog No. ABIN7237648

Product Details anti-GPR139 Antibody

Target: GPR139 (G Protein-Coupled Receptor 139 (GPR139))

Binding Specificity: AA 204-217, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR139 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: A Rabbit Polyclonal antibody to Parkin

Specificity: Intracellular, 3rd loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,Human - identical

Characteristics: Intracellular, 3rd loop

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RKSNFRLRGYSTGK, corresponding to amino acid residues 204 - 217 of mouse GPR139

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200-1:800

Comment:

Negative Control: BLP-GR069

Blocking Peptide: BLP-GR069

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR139

Target: GPR139 (G Protein-Coupled Receptor 139 (GPR139))

Alternative Name: GPR139  (GPR139 Products)

Background: G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1,G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs are a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR139 is an orphan GPCRs for which amino acids L-tryptophan and L-phenylalanine, the adrenocorticotropic hormone (ACTH), and α- and β-melanocyte-stimulating hormone have been proposed as endogenous agonists3. GPR139 was found to be highly conserved across species, as the amino acid sequence of the human GPR139 protein is 96 % , 92 % and 70 % identical to the mouse, chicken and zebrafish orthologs, respectively. According to mRNA expression levels GPR139 is mainly expressed in the central nervous system (CNS). GPR139 has been reported to activate several G protein pathways of which Gq/11 is the primary one4.

Alternative names: G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1

Gene ID: 209776

NCBI Accession: NM_001002911

UniProt: Q80UC8