VDAC1 antibody (N-Term)

Catalog No. ABIN7540555

Supplier Product No.: 600-101-hb2

Product Details anti-VDAC1 Antibody

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 600-101-hb2

Supplier: Rockland

Purpose: VDAC/Porin Antibody

Cross-Reactivity (Details): Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein.  

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: VDAC/Porin Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal portion of Human VDAC1/Porin1.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

VDAC/Porin Antibody has been tested for use in ELISA, western blot, and Immunofluorescence.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract. Positive control used in WB: HeLa Whole Cell, Mouse Heart Lysate, or Rat Brain Tissue Lysate, and HeLa Cells in IF.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store VDAC antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-VDAC1 antibody (ABIN7540555)

Vo, Choi, Choi: "The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagy." in: PLoS pathogens, Vol. 19, Issue 7, pp. e1011548, (2023) (PubMed).

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC1 (VDAC1 Products)

Background: Goat anti-VDAC/Porin antibody, VDAC-1 Loading Control Antibody, Porin Loading Control Antibody, VDAC-1 Antibody, VDAC1 Antibody, POR1 Antibody, Porin Antibody, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL,VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger).  The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane.  VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.  VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.  Diseases associated with VDAC1 include Cystic Fibrosis and Gerstmann-Straussler Disease. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations. Anti-VDAC/Porin Antibody is useful for researchers interested in Apoptosis, cell survival and regulation, Mitochondrial Proteins, and Neuroscience Research.

Gene ID: 7416

NCBI Accession: NP_003365

UniProt: P21796