SYCP3 antibody (N-Term)

Catalog No. ABIN7540636

Supplier Product No.: 600-401-mp9s

Product Details anti-SYCP3 Antibody

Target: SYCP3 (Synaptonemal Complex Protein 3 (SYCP3))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYCP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-mp9s

Supplier: Rockland

Purpose: Scp3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Scp3.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Scp3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal region of mouse Scp3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-Scp3 Antibody has been tested in ELISA, WB, and IHC.  Positive control used: Mouse Testis Lysate (p/n W10-000-GZ2) in Western Blot, Mouse Testis in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SYCP3

Target: SYCP3 (Synaptonemal Complex Protein 3 (SYCP3))

Alternative Name: Sycp3 (SYCP3 Products)

Background: Rabbit Anti-Synaptonemal complex protein 3 Antibody, SCP-3, Sycp3, Scp3, Synaptonemal complex protein 3,Synaptonemal complex protein 3 (Scp3) is a component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. These proteins are required for centromere pairing during meiosis in male germ cells. It is required for normal meiosis during spermatogenesis and male fertility but plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Anti-Scp3 Antibody is useful for researchers interested in Meiosis, Mitotic Cell Cycle, and fertility.

Gene ID: 20962

NCBI Accession: NP_035647

UniProt: P70281