CBP antibody (N-Term)

Catalog No. ABIN7540638

Supplier Product No.: 600-401-mq0s

The Rabbit Polyclonal anti-CBP antibody has been validated for WB and ELISA. It is suitable to detect CBP in samples from Human.

Quick Overview for CBP antibody (N-Term) (ABIN7540638)

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CBP Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-mq0s

Supplier: Rockland

Purpose: CREBBP Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human CREBBP.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-CREBBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal portion of human CREBBP conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-CREBBP Antibody has been tested in ELISA and WB.  Expect a band ~265kDa in western blot using appropriate tissue or lysates.  Positive control used: Mouse brain, MOLT-4, A549, and PC3 lysates in Western Blots.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CBP

Target: CBP (CREBBP) (CREB Binding Protein (CREBBP))

Alternative Name: CREBBP

Background: Rabbit Anti-CREBBP Antibody, Rabbit Anti-CREB Binding Protein, CREB-Binding Protein, EC 2.3.1.48, CBP, Rubinstein-Taybi Syndrome, KAT3A, MKHK1, RSTS1, RSTS,CREBBP (CREB Binding Protein) is ubiquitously expressed. It acetylates histones, giving a specific tag for transcriptional activation and acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes, so it's known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP acts as a coactivator of ALX1 and acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It acetylates PCNA, in which this acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Bromodomains (BRDs) are epigenetic reader domains that selectively recognize acetylated lysine residues on the tails of histone proteins and are the only known protein modules that can target acetylated lysine residues. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS) and acute myeloid leukemia. Anti-CREBBP Antibody is useful for researchers interested in Transcription Factors, Histones, Epigenetics, and Cancer research.

Gene ID: 1387

UniProt: Q92793

Pathways: TCR Signaling, Interferon-gamma Pathway, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha