T4 RNA Ligase antibody

Catalog No. ABIN7540667

Supplier Product No.: 200-401-mx4

Product Details

Target: T4 RNA Ligase

Reactivity: Bacteriophage T4

Host: Rabbit

Clonality: Polyclonal

Application: ELISA

Supplier Product No.: 200-401-mx4

Supplier: Rockland

Purpose: T4 RNA Ligase Antibody

Cross-Reactivity (Details): Protein A purified T4 RNA Ligase antibody is directed against T4 RNA Ligase (E.coli)

Sterility: Sterile filtered

Immunogen: Anti-T4 RNA Ligase antibody was produced from whole rabbit serum produced by repeated immunizations with T4 Ligase isolated from E. coli.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:13,000-1:14,000

Comment:

This protein A purified Anti-T4 RNA Ligase antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43.5 kDa in size corresponding to RNA Ligase by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: T4 RNA Ligase

Background: Enterobacteria phage T4, RNA ligase 1, polyribonucleotide synthase (ATP), ribonucleic ligase,The T4 RNA Ligase enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA. This catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[RNA]. Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in the formation of the phosphodiester bond and release of the adenylate.

Gene ID: 258717

NCBI Accession: NP_049839

UniProt: A0A7S9SW04