Env (ENV) antibody
ENV
Reactivity: Mouse Mammary Tumor Virus (MMTV)
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- Env (ENV)
- Reactivity
- Mouse Mammary Tumor Virus (MMTV)
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
-
Application
- ELISA, Western Blotting (WB)
- Purpose
- MMTV Antibody
- Characteristics
- Synonyms: rabbit anti-MMTV Antibody, rabbit anti-mouse mammary tumor virus surface protein antibody, Envelope glycoprotein gp70, Surface protein, Glycoprotein 52, Transmembrane protein, Glycoprotein 36, Mouse mammary tumor virus (strain GR) (MMTV)
- Purification
- Mouse mammary tumor virus surface protein antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01 % and cross adsorbed with 6X HIS.
- Sterility
- Sterile filtered
- Immunogen
-
Immunogen: MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His.
Immunogen Type: Native Protein
-
-
- Application Notes
- Application Note: Anti-MMTV Antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 41.2 kDa in size corresponding to MMTV surface protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:2000 ELISA Dilution: 1:10,000-1:20,000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 66 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target
- Env (ENV)
- Alternative Name
- env
- Target Type
- Viral Protein
- Background
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI) and is suitable for Cancer, Enzyme, and Viral research. Mouse mammary tumor virus surface protein binds the virus to the receptor of the host cells triggering transmembrane protein refolding. This activates the fusion peptide and the structure coils and repeats positioning the fusion peptide near the C-terminus region. Inturn increasing close proximity and then fusion of viral and cell membranes. Anti-MMTV is ideal for researchers interested in Cancer, Viral, and Enzyme research.
- UniProt
- P03374
-
You are here:
