Recombinant Myc Tag antibody (AA 408-439)

Catalog No. ABIN7581767

This Rabbit Monoclonal antibody specifically detects Myc Tag in WB, ELISA, IF and FACS. It exhibits reactivity toward Tag.

Quick Overview for Recombinant Myc Tag antibody (AA 408-439) (ABIN7581767)

Target: Myc Tag

Antibody Type: Recombinant Antibody

Reactivity: Tag

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Animal-Free

Clone: TUN219-2C1 (9E10)

Product Details anti-Myc Tag Antibody

Binding Specificity: AA 408-439

Expression System: Phage display

Purpose: Rabbit Anti-c-myc Antibody, animal-free mAb

Specificity: This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10

Cross-Reactivity (Details): No known cross reactivity. No binding to native c-Myc protein.

Characteristics: This antibody can be detected with anti-rabbit Fc secondary antibodies.

Purification: Protein A purification

Immunogen: No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4,

Storage: -20 °C

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: c-myc tag

Target Type: Tag

Background: Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.

Molecular Weight: 1.2 kDa