Recombinant Myc Tag antibody (AA 408-439)
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- Target See all Myc Tag products
- Myc Tag
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 408-439
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Reactivity
- Tag
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Host
- Rabbit
- Expression System
- Phage display
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Clonality
- Monoclonal
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Conjugate
- This Myc Tag antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Rabbit Anti-c-myc Antibody, animal-free mAb
- Specificity
- This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10
- Cross-Reactivity (Details)
- No known cross reactivity. No binding to native c-Myc protein.
- Characteristics
- This antibody can be detected with anti-rabbit Fc secondary antibodies.
- Purification
- Protein A purification
- Grade
- Animal-Free
- Immunogen
- No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc
- Clone
- TUN219-2C1 (9E10)
- Isotype
- IgG
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anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal unconjugated
anti-Myc Tag (C-Term) antibodyReactivity: Human WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC Host: Mouse Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (Biotin)Reactivity: Human WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC Host: Mouse Monoclonal 9E10 Biotin
anti-Myc Tag (C-Term) antibody (FITC)Reactivity: Human FACS, ICFC Host: Mouse Monoclonal 9E10 FITC
anti-Myc Tag antibodyWB, IP, ELISA, IF Host: Mouse Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA Host: Mouse Monoclonal 9E10- unconjugated
anti-Myc Tag antibodyWB, IP, IF Host: Mouse Monoclonal 3B8 unconjugated
anti-Myc Tag (AA 410-419) antibodyWB, IP, IF, IHC, ICC Host: Mouse Monoclonal Myc-A7 unconjugated
anti-Myc Tag (AA 410-419) antibodyWB Host: Goat Polyclonal unconjugated
anti-Myc Tag (AA 408-439), (C-Term) antibodyIF, IHC, FACS, StM Host: Mouse Monoclonal 9E10-3 unconjugated
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- Application Notes
- Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4,
- Storage
- -20 °C
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- Target
- Myc Tag
- Alternative Name
- c-myc tag (Myc Tag Products)
- Target Type
- Tag
- Background
- Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.
- Molecular Weight
- 1.2 kDa
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