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Recombinant Myc Tag antibody (AA 408-439)

Name: Recombinant Myc Tag antibody (AA 408-439)
Brand: antibodies-online
SKU: ABIN7581767
Price: 700.55 USD
Availability: InStock

Animal-Free Reactivity: Tag WB, ELISA, IF, FACS Host: Rabbit Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

Quantitative immunodetection of a myc tagged protein using the automated Protein Simple Western Immunoassay system. (Recombinant Myc Tag antibody (AA 408-439))

Whole mount of 2 dpf transgenic zebrafish expressing in the CNS the myc-epitope as a fusion to a fluorescent protein stained with recombinant Hyper-Myc Mouse and fluorescently labelled secondary antiy-mouse Fc antibody (Recombinant Myc Tag antibody (AA 408-439))

Improvement of affinitiy and Fv-region thermal stability of Hyper-Myc compared to hybridoma antibody Myc1- 9E10 recognizing the myc-epitope (Recombinant Myc Tag antibody (AA 408-439))

BLI

ELISA

4 images

Catalog No. ABIN7581767

Datasheet as PDF

Target See all Myc Tag products

Myc Tag

Antibody Type

Recombinant Antibody
Binding Specificity
24

21

12

8

5

3

2

1
AA 408-439
Reactivity
34

5

3
Tag
Host
76

50

10

9

6

5

1

1

1
Rabbit
Compatible Secondaries

Expression System

Phage display
Clonality
89

58

10
Monoclonal
Conjugate
80

13

9

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is un-conjugated
Application
121

63

49

44

35

34

20

19

15

11

11

6

6

5

4

4

4

3

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose

Rabbit Anti-c-myc Antibody, animal-free mAb

Specificity

This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10

Cross-Reactivity (Details)

No known cross reactivity. No binding to native c-Myc protein.

Characteristics

This antibody can be detected with anti-rabbit Fc secondary antibodies.

Purification

Protein A purification

Grade

Animal-Free

Immunogen

No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc

Clone

TUN219-2C1 (9E10)

Isotype

IgG
anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reactivity: Human FACS Host: Mouse Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Host: Mouse Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Host: Mouse Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal Myc-A7 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB Host: Goat Polyclonal unconjugated

anti-Myc Tag (AA 408-439), (C-Term) antibody
IF, IHC, FACS, StM Host: Mouse Monoclonal 9E10-3 unconjugated

Application Notes

Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

PBS, pH 7.4,

Storage

-20 °C
Target

Myc Tag

Alternative Name

c-myc tag (Myc Tag Products)

Target Type

Tag

Background

Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.

Molecular Weight

1.2 kDa