TMEM150C antibody (Extracellular)

Catalog No. ABIN7581992

Product Details anti-TMEM150C Antibody

Target: TMEM150C (Transmembrane Protein 150C (TMEM150C))

Binding Specificity: AA 33-50, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM150C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal antibody to TMEM150C/Tentonin 3 (extracellular).

Sequence: (C)EDDKILPLNS AARKSGVK

Specificity: Extracellular, 1st loop.

Predicted Reactivity: Mouse - 17 out of 18 amino acid residues identical Human - 16 out of 18 amino acid residues identical

Characteristics: Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (ABIN7581992) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM150C from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EDDKILPLNSAARKSGVK, corresponding to amino acid residues 33 - 50 of rat TMEM150C

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMEM150C

Target: TMEM150C (Transmembrane Protein 150C (TMEM150C))

Alternative Name: TMEM150C (TMEM150C Products)

Background: Transmembrane Protein 150C, TTN3,Transmembrane protein 150C (TMEM150C) also known as Tentonin-3 (TTN3), is a small protein composed of 249 amino acids comprising six transmembrane domains.TMEM150C which was first thought to be a mechanosensitive ion channel that confers a relatively slow inactivating current in proprioceptive neurons in mouse dorsal root ganglion (DRG)1. Later studies, however, showed that the mechanosensitive currents attributed to TMEM150C in different cell setups, could be attributed to the presence of endogenous Piezo1 channels2.Although, some controversy still remains, it is generally accepted that TMEM150C does not form a mechanosensitive channel per se, but rather it functions as a general regulator or sensor, of mechanosensitive channels like Piezo1, Piezo2 and TREK1 (K2P2.1) 2,3.TMEM150C has also been described in the aortic arch and nodose ganglia neurons, where it is involved in the mechanotransduction of arterial pressure to electrical signals in baroreceptors. Dysregulation of TMEM150C alters the sensitivity of the baroreflex, a cardiovascular reflex that is essential for blood pressure homeostasis, leading to diseases such as hypertension, stroke, and heart failure 4.Furthermore, recent evidence suggests that TMEM150C functions as a stretch-activated cation channel in pancreatic β cells, contributing to insulin secretion induced by high glucose levels and hypotonicity. Whether TMEM150C functions in this context as a stretch activated channel itself or whether it functions in conjunction with other proposed stretch-activated channels like TRPV4 or TRPV2, remains to be seen 3,5.

Gene ID: 360916

UniProt: B5DFH9