Human Papilloma Virus 16 E6 (HPV-16 E6) (AA 85-158) antibody

Catalog No. ABIN730618

Product Details

Target: Human Papilloma Virus 16 E6 (HPV-16 E6)

Binding Specificity: AA 85-158

Reactivity: Human Papillomavirus 16 (HPV-16), Virus, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Virus

Cross-Reactivity (Details): HPV16

Purification: Purified by Protein A.

Immunogen: synthetic peptide from HPV16-E6 conjugated to KLH

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Shao, Sun, Wang, Chung, Du, Wang, Qiu, Wang, Wu: "HPV16 E6/E7 upregulates HIF-2α and VEGF by inhibiting LKB1 in lung cancer cells." in: Tumour biology, Vol. 39, Issue 7, pp. 1010428317717140, (2017) (PubMed).

Yang, Li, Wang, Hou, Qiu, Wang, Wu: "Long-term persistent infection of HPV 16 E6 up-regulate SP1 and hTERT by inhibiting LKB1 in lung cancer cells." in: PLoS ONE, Vol. 12, Issue 8, pp. e0182775, (2017) (PubMed).

Target Details

Target: Human Papilloma Virus 16 E6 (HPV-16 E6)

Alternative Name: HPV16 E6 protein

Target Type: Viral Protein

Background:

Synonyms: E6, HPV16 E6 protein, Human Papilloma Virus, Human papillomavirus type 16 E6, Human papillomavirus type 16, Protein E6, E6 protein, HPV16 E6, HPV16-E6, VE6_HPV16.

Background: Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.

Gene ID: 1489078

UniProt: P03126