CD33 antibody (CD33) (APC) Primary Antibody
CD33 Reactivity: Human, Non-Human Primate FACS, Func, ICC, IHC (f), IP, MCA, WB Host: Mouse Monoclonal WM53
Catalog No. ABIN775711
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- Human, Non-Human Primate
- This CD33 antibody is conjugated to APC
- Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA), Western Blotting (WB)
- The mouse monoclonal antibody WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV, WS Code M-505
- Cross-Reactivity (Details)
- Human, Non-Human Primates
- Human AML cells
- Application Notes
- The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- For Research Use only
- No reconstitution is necessary.
- The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
Avoid prolonged exposure to light.
- 4 °C
- Storage Comment
- Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Garnache-Ottou, Chaperot, Biichle, Ferrand, Remy-Martin, Deconinck, de Tailly, Bulabois, Poulet, Kuhlein, Jacob, Salaun, Arock, Drenou, Schillinger, Seilles, Tiberghien, Bensa, Plumas, Saas: "Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells." in: Blood, Vol. 105, Issue 3, pp. 1256-64, 2005 (PubMed).
Leone, Rutella, Bonanno, Abbate, Rebuzzi, Giovannini, Lombardi, Galiuto, Liuzzo, Andreotti, Lanza, Contemi, Leone, Crea: "Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function." in: European heart journal, Vol. 26, Issue 12, pp. 1196-204, 2005 (PubMed).
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Vitale, Romagnani, Puccetti, Olive, Costello, Chiossone, Pitto, Bacigalupo, Moretta, Mingari: "Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 10, pp. 5764-9, 2001 (PubMed).
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Bradstock, Kirk, Grimsley, Kabral, Hughes: "Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations." in: British journal of haematology, Vol. 72, Issue 4, pp. 512-8, 1989 (PubMed).
Favaloro, Bradstock, Kabral, Grimsley, Zowtyj, Zola et al.: "Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging ..." in: British journal of haematology, Vol. 69, Issue 2, pp. 163-71, 1988 (PubMed).
Favaloro, Bradstock, Kabral, Grimsley, Berndt: "Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33)." in: Disease markers, Vol. 5, Issue 4, pp. 215-25, 1988 (PubMed).
- Garnache-Ottou, Chaperot, Biichle, Ferrand, Remy-Martin, Deconinck, de Tailly, Bulabois, Poulet, Kuhlein, Jacob, Salaun, Arock, Drenou, Schillinger, Seilles, Tiberghien, Bensa, Plumas, Saas: "Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells." in: Blood, Vol. 105, Issue 3, pp. 1256-64, 2005 (PubMed).
- Alternative Name
- CD33 (CD33 Antibody Abstract)
- SIGLEC-3, SIGLEC3, p67, Siglec-3, gp67, CD33 molecule, CD33 antigen, CD33, Cd33
- CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
- Gene ID
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