Lhcii Type III Chlorophyll A/B-Binding Protein (Lhcb3) antibody

Details for Product No. ABIN93508
Antigen
Reactivity
Arabidopsis thaliana, Barley (Hordeum vulgare), Green bean (Phaseolus vulgaris), Maize/Corn (Zea mays), Pea (Pisum sativum), Rice (Oryza sativa), Spinach (Spinacia oleracea), Tobacco (Nicotiana tabacum)
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Options
Immunogen BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb3 UniProt: Q9S7M0,TAIR:At5g54270. This sequence is highly conserved even in Ginko biloba and one of the major LHCII-forms of Physcomitrella patens.
Isotype IgG
Characteristics Expected / apparent Molecular Weight of the Antigene: 24 / 26 kDa for Arabidopsis thaliana
Purification affinity purified
Antigen
Background AGI Code: At5g54270
The major light-harvesting antenna complex II (LHCII) in photsynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. While Lhcb1 and Lhcb2 are quite similar and regularily present in multiple gene-copies, the Lhcb3 protein differs in pigment-composition and molecular size and often is coded by only a single gene. Lhcb3 seems not to be present in the mobile LHCII trimers involved in state 1-state 2 transitions. A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476.
Molecular Weight expected: 24 kDa, apparent: 26 kDa for Arabidopsis thaliana
UniProt Q9S7M0
Application Notes Recommended Dilution: 1 : 2000 with standard ECL (WB).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 200 µL of sterile water
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Product cited in: Jung, Lee, Lee, Kang, Kim, Grimm, Back: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, 2008 (PubMed).

Tikkanen, Nurmi, Suorsa, Danielsson, Mamedov, Styring, Aro: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, 2008 (PubMed).