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Lhca4 antibody

Lhca4 Reactivity: Arabidopsis thaliana, Barley, Moss, Nicotiana tabacum, Oryza sativa, Spinach, Zea mays WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN93514
  • Target
    Lhca4 (psi Type IV Chlorophyll A/B-Binding Protein (Lhca4))
    Reactivity
    Arabidopsis thaliana, Barley, Moss, Nicotiana tabacum, Oryza sativa, Spinach, Zea mays
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 22 / 21 kDa for Arabidopsis thaliana
    Purification
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.
    Isotype
    IgG
  • Application Notes
    Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
  • Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

    Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

  • Target
    Lhca4 (psi Type IV Chlorophyll A/B-Binding Protein (Lhca4))
    Background
    AGI Code: At3g47470
    The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.
    Molecular Weight
    expected: 22 kDa, apparent: 21 kDa for Arabidopsis thaliana
    UniProt
    P27521
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