Lhca4 antibody

Catalog No. ABIN93514

Product Details anti-Lhca4 Antibody

Target: Lhca4 (psi Type IV Chlorophyll A/B-Binding Protein (Lhca4))

Reactivity: Spinach, Zea mays, Arabidopsis thaliana, Barley, Moss, Nicotiana tabacum, Oryza sativa

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 22 / 21 kDa for Arabidopsis thaliana

Purification: affinity purified

Immunogen: BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Lhca4 antibody (ABIN93514)

Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Target Details for Lhca4

Target: Lhca4 (psi Type IV Chlorophyll A/B-Binding Protein (Lhca4))

Background: AGI Code: At3g47470
The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.

Molecular Weight: expected: 22 kDa, apparent: 21 kDa for Arabidopsis thaliana

UniProt: P27521