Chlorophyll A-B Binding Protein CP26 (LHCB5) antibody

Details for Product No. ABIN93515
Antigen
Reactivity
Arabidopsis thaliana, Barley (Hordeum vulgare), Rice (Oryza sativa), Pea (Pisum sativum), Spinach (Spinacia oleracea), Maize/Corn (Zea mays)
Host
Rabbit
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen BSA-conjugated synthetic peptide derived from Arabidosis thaliana Lhcb5 protein UniProt: Q9XF89, TAIR: At4g10340 sequence. This sequence is highly conserved in Lhcb5 proteins from monocots, dicots and conifers but only partial conserved in Physcomitrella patens and Chlamydomonas reinhardtii.
Isotype IgG
Cross-Reactivity (Details) Peptide target used to elicit this antibody has a weak, sporadic conservation withGlutamine Synthetase to III, antibody not expected to detect this enzyme. Weak conservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase)(GLNRS), but antibody not expected to detect this enzyme.
Characteristics Expected / apparent Molecular Weight of the Antigene: 26 / 25-26 kDa for Arabidopsis thaliana
Purification affinity purified
Antigen
Alternative Name CP26 chlorophyll a/b-binding protein of plant PSII (Lhcb5)
Background AGI Code: At4g10340
Lhcb5 is one of the 3 minor highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
Molecular Weight expected: 26 kDa, apparent: 25-26 kDa for Arabidopsis thaliana
UniProt Q9XF89
Application Notes Recommended Dilution: 1 : 1000, detected with standard ECL (WB).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 200 µL of sterile water
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Chlorophyll A-B Binding Protein CP26 (LHCB5) antibody (ABIN93515) Arabidopsis thaliana,chloroplasts(1), Arabidopsis thaliana,thylakoids (2), Arabidopsi...
Product cited in: Zienkiewicz, Ferenc, Wasilewska, Romanowska: "High light stimulates Deg1-dependent cleavage of the minor LHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana." in: Planta, Vol. 235, Issue 2, pp. 279-88, 2012 (PubMed).

Jung, Lee, Lee, Kang, Kim, Grimm, Back: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, 2008 (PubMed).