D1 Protein of PSII, (PsbA) (C-Term) antibody

Details for Product No. ABIN93523
Antigen
Epitope
C-Term
1
1
Reactivity
Amphidinium carterae, Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii), Chlamydomonas raudensis, Gonyaulax polyedra, Fucus vesiculosus, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Lobaria pulmonaria, Pinus sylvestris, Stony Coral (Turbinaria)
3
3
1
1
1
Host
Chicken
3
Clonality
Polyclonal
Application
Western Blotting (WB)
3
Options
Immunogen KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
Isotype IgY
Specificity The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases. Peptide used for antibody product.
Characteristics Expected / apparent Molecular Weight of the Antigene: 38 / 28-30 kDa
Purification affinity purified
Antigen
Background AGI Code: ATCG00020
The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
Molecular Weight expected: 38 kDa, apparent: 28-30 kDa
UniProt P14660, P0C434, P83755, Q14FH6
Application Notes Recommended Dilution: 1 : 4000 - 1 : 8000 on 5 µg of total protein, detected with standard ECL (WB).
A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cellsprior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies willnot detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.
Comment

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.

Restrictions For Research Use only
Format Liquid
Buffer PBS pH 8.0+ 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Make aliquots to avoid working with a stock.
Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubek.
Storage 4 °C
Supplier Images
Image no. 1 for anti-D1 Protein of PSII, (PsbA) (C-Term) antibody (ABIN93523) anti-D1 Protein of PSII, (PsbA) (C-Term) antibody (Image 1)
Image no. 2 for anti-D1 Protein of PSII, (PsbA) (C-Term) antibody (ABIN93523) Following lanes contained whole cell extracts from (1.) Higher plants - Spinach, (2.)...
Image no. 3 for anti-D1 Protein of PSII, (PsbA) (C-Term) antibody (ABIN93523) anti-D1 Protein of PSII, (PsbA) (C-Term) antibody (Image 3)
Product cited in: Muktiono, Schulten, Heemken, Gandrass, Prange, Schnabl, Cerboncini: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." in: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, 2008 (PubMed).

Garczarek, Dufresne, Blot, Cockshutt, Peyrat, Campbell, Joubin, Six: "Function and evolution of the psbA gene family in marine Synechococcus: Synechococcus sp. WH7803 as a case study." in: The ISME journal, Vol. 2, Issue 9, pp. 937-53, 2008 (PubMed).

Drath, Kloft, Batschauer, Marin, Novak, Forchhammer: "Ammonia triggers photodamage of photosystem II in the cyanobacterium Synechocystis sp. strain PCC 6803." in: Plant physiology, Vol. 147, Issue 1, pp. 206-15, 2008 (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, 2008 (PubMed).

Kuepper, Setlik, Seibert, Prasil, Setlikova, Strittmatter, Levitan, Lohscheider, Adamska, Berman-Frank: "Iron limitation in the marine cyanobacterium Trichodesmium reveals new insights into regulation of photosynthesis and nitrogen fixation." in: The New phytologist, Vol. 179, Issue 3, pp. 784-98, 2008 (PubMed).

Takahashi, Whitney, Itoh, Maruyama, Badger: "Heat stress causes inhibition of the de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 11, pp. 4203-8, 2008 (PubMed).