BrdU antibody (Bromodeoxyuridine)

Details for Product anti-BrDU Antibody No. ABIN93883
Antigen
Reactivity
139
91
29
5
4
3
1
1
1
Host
Mouse
256
37
24
13
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This BrdU antibody is un-conjugated
21
8
5
5
4
4
4
4
4
4
3
3
3
3
3
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
162
146
122
107
39
36
26
25
24
21
21
10
10
2
2
2
1
1
1
1
1
Options
Immunogen 5-bromodeoxyuridine conjugated with hemocyanine.
Clone MoBu-1
Isotype IgG1
Specificity The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name 5-bromodeoxyuridine (BrdU)
Target Type Chemical
Background Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Bromodeoxyuridine (BrDU) antibody (ABIN93883) Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute...
Image no. 2 for anti-Bromodeoxyuridine (BrDU) antibody (ABIN93883) Immunohistochemistry of paraffin-embedded sections (chick embryo) Immunohistochemistr...
Product cited in: Babic, Ondrejcakova, Bakos, Racekova, Jezova: "Cell proliferation in the hippocampus and in the heart is modified by exposure to repeated stress and treatment with memantine." in: Journal of psychiatric research, Vol. 46, Issue 4, pp. 526-32, 2012 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:500). Further details: The sections were incubated with mouse monoclonal anti-BrdU antibody (1:500) at 4 degrees Celsius overnight.

Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." in: European journal of cell biology, Vol. 79, Issue 3, pp. 202-7, 2000 (PubMed).

Buckiová, Kubínová, Soukup, Jelínek, Brown: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." in: The International journal of developmental biology, Vol. 42, Issue 5, pp. 737-40, 1998 (PubMed).