Glycophorin A antibody (Glycophorin A (MNS Blood Group)) (N-Term)

Details for Product anti-GYPA Antibody No. ABIN94041
Antigen
Epitope
N-Term
28
6
4
3
2
2
2
2
2
2
2
1
1
Reactivity
Human
408
34
17
15
Host
Mouse
258
130
31
7
Clonality (Clone)
Monoclonal ()
Conjugate
This Glycophorin A antibody is un-conjugated
47
36
20
14
6
6
6
6
6
6
6
6
6
6
6
6
6
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Application
Aggregation (AGG), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
294
147
106
79
73
44
34
22
22
14
11
7
6
5
4
4
2
1
Options
Immunogen Synthetic peptide (Human, N-terminal)
Clone HIR2
Isotype IgG2b
Specificity The antibody HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
HLDA VII, WS Code 70299
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD235a (GYPA Antibody Abstract)
Background CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
Pathways Maintenance of Protein Location
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Glycophorin A (MNS Blood Group) (GYPA) (N-Term) antibody (ABIN94041) Surface staining of human peripheral blood with anti-CD235a (HIR2) purified, GAM-APC.