CD31 antibody (Platelet/endothelial Cell Adhesion Molecule)

Details for Product anti-PECAM1 Antibody No. ABIN94069
Antigen
Reactivity
Human
908
273
142
104
103
32
21
17
11
11
8
8
7
2
2
2
2
2
1
1
1
1
1
Host
Mouse
688
318
110
8
5
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD31 antibody is un-conjugated
87
81
79
37
17
16
11
11
11
10
10
9
9
9
9
9
9
9
9
9
9
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9
9
8
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5
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
697
483
354
348
157
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99
97
59
33
25
20
17
15
13
11
10
8
3
2
1
1
1
1
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1
Options
Immunogen Leukocytes of patient suffering from LGL-type leukaemia
Clone MEM-05
Isotype IgG1
Specificity The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD31 (PECAM1 Antibody Abstract)
Background CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
Pathways Regulation of Actin Filament Polymerization
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody (ABIN94069) Surface staining of lysed and wash human peripheral blood with purified anti-human CD...
Product cited in: Prager, Staffler, Majdic, Säemann, Godár, Zlabinger, Stockinger: "Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 4, pp. 2364-71, 2001 (PubMed).