CD33 antibody (CD33 Molecule) (Biotin)

Details for Product anti-CD33 Antibody No. ABIN94073
Antigen
Reactivity
Human, Non-Human Primate
472
49
38
22
7
7
4
2
1
Host
Mouse
346
149
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD33 antibody is conjugated to Biotin
55
52
32
23
9
7
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
308
194
104
100
89
50
28
26
22
19
19
16
12
6
6
5
3
2
2
1
Options
Immunogen NFMY-9s human cell line
Clone HIM3-4
Isotype IgG1
Specificity The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA V, WS Code M MA112
HLDA VI, WS Code M MA47
Cross-Reactivity (Details) Human, Non-Human Primates
Antigen
Alternative Name CD33 (CD33 Antibody Abstract)
Background CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD33 Molecule (CD33) antibody (Biotin) (ABIN94073) Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) F...
Product cited in: McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, 2013 (PubMed).