CD45RB (CD45RB) antibody

Details for Product No. ABIN94146
Antigen
Reactivity
Human
212
74
16
6
5
4
3
1
Host
Mouse
194
77
19
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
31
27
19
15
6
6
6
6
6
6
6
6
6
6
6
6
6
4
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
265
138
121
89
54
53
17
16
7
3
2
1
Options
Immunogen Human peripheral blood lymphocytes.
Clone MEM-143
Isotype IgG1
Specificity The antibody MEM-143 reacts with a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD45RB (CD45RB Antibody Abstract)
Background CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD45RB (CD45RB) antibody (ABIN94146) Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD45RB (...
Product cited in: Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, 2011 (PubMed).