CD45RB (CD45RB) antibody (PE)

Details for Product No. ABIN94143
Antigen
Reactivity
Human, Non-Human Primate
212
74
16
6
5
3
3
1
Host
Mouse
194
77
19
Clonality (Clone)
Monoclonal ()
Conjugate
PE
31
26
19
15
6
6
6
6
6
6
6
6
6
6
6
6
6
4
3
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
265
138
121
88
54
52
17
16
7
3
2
1
Options
Immunogen Human thymocytes and T lymphocytes.
Clone MEM-55
Isotype IgG1
Specificity The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
HLDA III, WS Code NL 358
HLDA IV, WS Code NL 2
HLDA V, WS Code T T-151
HLDA V, WS Code T T-CD45.08
Cross-Reactivity (Details) Human, Non-Human Primates
Antigen
Alternative Name CD45RB (CD45RB Antibody Abstract)
Background CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD45RB (CD45RB) antibody (PE) (ABIN94143) Surface staining of human peripheral blood with anti-CD45RB (MEM-55) PE.
Product cited in: Koethe, Zander, Köster, Annan, Ebenfelt, Spencer, Bemark: "Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation." in: Journal of leukocyte biology, Vol. 90, Issue 1, pp. 5-19, 2011 (PubMed).