CD80 antibody (CD80) (Extracellular Domain) (FITC)

Details for Product anti-CD80 Antibody No. ABIN94240, Supplier: Log in to see
Antigen
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Alternatives
anti-Human CD80 antibody for Immunoprecipitation
Epitope
Extracellular Domain
32
24
15
15
15
14
12
11
11
10
8
7
6
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
455
286
49
11
11
6
3
3
2
2
1
Host
Mouse
398
126
97
94
42
16
Clonality (Clone)
Monoclonal ()
Conjugate
This CD80 antibody is conjugated to FITC
70
70
53
40
19
17
16
15
10
7
7
6
6
6
6
6
6
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
508
220
171
153
114
101
81
42
40
26
22
22
12
8
8
6
6
5
5
5
2
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Extracellular domain of human CD80 fused to human IgG1(Fc)
Clone MEM-233
Isotype IgG1
Specificity The antibody MEM-233 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Plasmids, Primers & others Plasmids, Primers & others CD80 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD80 (CD80) (Extracellular Domain) antibody (FITC) (ABIN94240) Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80 (MEM-2...
Product cited in: Silk, Leishman, Nishimoto, Reddy, Fairchild: "Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 172420, 2012 (PubMed).

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, 2007 (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007 (PubMed).

Campioni, Moretti, Ferrari, Punturieri, Castoldi, Lanza: "Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment." in: Haematologica, Vol. 91, Issue 3, pp. 364-8, 2006 (PubMed).

Tan, Beutelspacher, Wang, McClure, Ritter, Lombardi, George: "Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 11, Issue 5, pp. 790-800, 2005 (PubMed).

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, 2003 (PubMed).

Background publications Yadav, Judkowski, Flodstrom-Tullberg, Sterling, Redmond, Sherman, Sarvetnick: "B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3631-9, 2004 (PubMed).

Vasilevko, Ghochikyan, Holterman, Agadjanyan: "CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA." in: DNA and cell biology, Vol. 21, Issue 3, pp. 137-49, 2002 (PubMed).