CD80 antibody (CD80) (Extracellular Domain)

Details for Product anti-CD80 Antibody No. ABIN94239
Antigen
Epitope
Extracellular Domain
23
15
15
15
12
11
9
9
8
7
7
6
5
5
4
4
4
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
325
219
39
11
9
6
3
2
1
1
1
Host
Mouse
243
152
92
40
39
7
Clonality (Clone)
Monoclonal ()
Conjugate
This CD80 antibody is un-conjugated
62
56
36
32
10
10
9
9
6
6
6
5
5
5
5
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
327
172
151
98
62
59
41
30
25
13
11
10
6
5
5
2
2
2
2
1
1
1
1
Options
Immunogen Extracellular domain of human CD80 fused to human IgG1(Fc)
Clone MEM-233
Isotype IgG1
Specificity The antibody MEM-233 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD80 (CD80) (Extracellular Domain) antibody (ABIN94239) Surface staining of RAJI human Burkitt lymphoma cell line with anti-human CD80 (MEM-2...
Product cited in: Silk, Leishman, Nishimoto, Reddy, Fairchild: "Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 172420, 2012 (PubMed).

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, 2007 (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007 (PubMed).

Campioni, Moretti, Ferrari, Punturieri, Castoldi, Lanza: "Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment." in: Haematologica, Vol. 91, Issue 3, pp. 364-8, 2006 (PubMed).

Tan, Beutelspacher, Wang, McClure, Ritter, Lombardi, George: "Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 11, Issue 5, pp. 790-800, 2005 (PubMed).

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, 2003 (PubMed).