Keratin 7/17 (KRT7/17) antibody

Details for Product No. ABIN94296
Antigen
Reactivity
Human, Pig (Porcine), Cow (Bovine)
15
10
4
Host
Mouse
16
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
16
10
9
7
5
3
2
2
2
1
1
Options
Immunogen Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Clone C-46
Isotype IgG1
Specificity The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
No Cross-Reactivity Mouse (Murine), Rat (Rattus), Rabbit
Cross-Reactivity (Details) Porcine, Human, Bovine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Cytokeratin 7+17 (KRT7/17 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Keratin 7/17 (KRT7/17) antibody (ABIN94296) Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody.
Image no. 2 for anti-Keratin 7/17 (KRT7/17) antibody (ABIN94296) Immunohistochemistry of paraffin-embedded sections (pancreas) Immunohistochemistry st...
Product cited in: Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, 1990 (PubMed).