MHC Class II HLA-DR1 antibody

Details for Product No. ABIN94350
Antigen
Reactivity
Human
10
Host
Mouse
10
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
10
9
9
Options
Immunogen Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
Clone MEM-267
Isotype IgG2b
Specificity The antibody MEM-267 specifically binds to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-DR1
Background HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-MHC Class II HLA-DR1 antibody (ABIN94350) Western blotting analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-...