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HLA-DR1 antibody

HLA-DR1 Reactivity: Human FACS, ELISA, WB Host: Mouse Monoclonal MEM-267 unconjugated

Separation of human HLA-DR1 positive lymphocytes (red-filled) from HLA-DR1 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human HLA-DR1 (empty) (MEM-267) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Anti-HLA-DR1 (empty) (clone MEM-267) works in WB application under reducing and non-reducing conditions.

Western blotting analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-267 antibody.

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human HLA-DR1 (empty) (MEM-267) purified antibody (concentration in sample 9 μg/mL, GAM APC).

4 images

Catalog No. ABIN94350

Datasheet as PDF

Target See all HLA-DR1 products

HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))
Reactivity
2

1
Human
Host
3
Mouse
Compatible Secondaries
Clonality
3
Monoclonal
Conjugate
3
This HLA-DR1 antibody is un-conjugated

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB)

Specificity

The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Clone

MEM-267

Isotype

IgG2b
anti-MHC Class II HLA-DR1 (HLA-DR1) antibody (PE)
HLA-DR1 Reactivity: Human FACS Host: Mouse Monoclonal MEM-267 PE

anti-MHC Class II HLA-DR1 (HLA-DR1) antibody (PE)
HLA-DR1 Reactivity: E. coli FACS Host: Mouse Monoclonal MEM-267 PE

anti-MHC Class II HLA-DR1 (HLA-DR1) antibody (PE)
HLA-DR1 Reactivity: Human FACS, ELISA, WB Host: Mouse Monoclonal MEM-267 PE

Application Notes

Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))

Alternative Name

HLA-DR1 (HLA-DR1 Products)

Background

HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.

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