Transferrin antibody (Tf)

Details for Product anti-Tf Antibody No. ABIN94486
Antigen
Reactivity
Human, Pig (Porcine), Rabbit
379
86
74
66
25
21
13
13
11
10
10
7
7
5
4
4
2
1
1
1
Host
Mouse
302
201
71
27
11
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Transferrin antibody is un-conjugated
58
56
30
10
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
328
289
153
83
72
69
62
62
30
29
28
28
24
21
18
14
11
9
8
8
6
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Options
Immunogen Purified porcine transferrin.
Clone HTF-14
Isotype IgG1
Specificity The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
No Cross-Reactivity Cow (Bovine), Sheep (Ovine), Dog (Canine), Horse (Equine)
Cross-Reactivity (Details) Human, Rabbit, Porcine
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Transferrin (Tf Antibody Abstract)
Background Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Pathways Transition Metal Ion Homeostasis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Transferrin (Tf) antibody (ABIN94486) Human transferrin detected by the mouse monoclonal antibody HTF-14. 1. hTransferrin; ...
Product cited in: Martens, Böttcher, Talbot, Bornscheuer, Abou-Hassan, Delcea: "Maghemite nanoparticles stabilize the protein corona formed with transferrin presenting different iron-saturation levels." in: Nanoscale, 2019 (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Bártek, Viklický, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, 1985 (PubMed).