Catalase antibody

Catalog No. ABIN96131

Supplier Product No.: 100-4151

Product Details anti-Catalase Antibody

Target: Catalase (CAT)

Reactivity: Human, Mouse, Rat, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 100-4151

Supplier: Rockland

Purpose: Catalase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Catalase [Bovine Liver].

Characteristics: Synonyms: rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody

Purification: Anti-Catalase Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen:

Immunogen: Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Catalase Antibody has been tested by western blotting and is suitable for IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:5,000 - 1:20,000

ELISA Dilution: 1:100,000 - 1:1,000,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Catalase Antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Catalase antibody (ABIN96131)

Colasuonno, Niceforo, Marioli, Fracassi, Stregapede, Massey, Tartaglia, Bertini, Compagnucci, Moreno: "Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency." in: Oxidative medicine and cellular longevity, Vol. 2020, pp. 6821247, (2020) (PubMed).

Lortz, Lenzen, Mehmeti: "Impact of scavenging hydrogen peroxide in the endoplasmic reticulum for β cell function." in: Journal of molecular endocrinology, Vol. 55, Issue 1, pp. 21-9, (2016) (PubMed).

Krysko, Stevens, Langenberg, Fransen, Espeel, Baes: "Peroxisomes in zebrafish: distribution pattern and knockdown studies." in: Histochemistry and cell biology, Vol. 134, Issue 1, pp. 39-51, (2010) (PubMed).

Miyata, Hosoi, Mukai, Fujiki: "In vitro import of peroxisome-targeting signal type 2 (PTS2) receptor Pex7p into peroxisomes." in: Biochimica et biophysica acta, Vol. 1793, Issue 5, pp. 860-70, (2009) (PubMed).

Rosicarelli, Stefanini: "DEHP effects on histology and cell proliferation in lung of newborn rats." in: Histochemistry and cell biology, Vol. 131, Issue 4, pp. 491-500, (2009) (PubMed).

Apraiz, Cajaraville, Cristobal: "Peroxisomal proteomics: biomonitoring in mussels after the Prestige's oil spill." in: Marine pollution bulletin, Vol. 58, Issue 12, pp. 1815-26, (2009) (PubMed).

Vincent, Kato, McLean, Soules, Feldman: "Sensory neurons and schwann cells respond to oxidative stress by increasing antioxidant defense mechanisms." in: Antioxidants & redox signaling, Vol. 11, Issue 3, pp. 425-38, (2009) (PubMed).

Drago, Giacomello, Pizzo, Pozzan: "Calcium dynamics in the peroxisomal lumen of living cells." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14384-90, (2008) (PubMed).

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: CAT (CAT Products)

Background: Background: Anti-Catalase Antibody detects catalase. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. Catalase is necessary in cells to protect them from the toxic effects of hydrogen peroxide and to promote growth of cells. Anti-Catalase antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Gene ID: 531682

UniProt: P00432

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism