Tetanus Toxin C-Fragment (TTC) antibody

Catalog No. ABIN964673

Supplier Product No.: 100-401-894

Product Details

Target: Tetanus Toxin C-Fragment (TTC)

Reactivity: Clostridium tetani

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 100-401-894

Supplier: Rockland

Purpose: Tetanus Toxin Fragment C Antibody

Cross-Reactivity (Details): This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani.

Characteristics: Synonyms: rabbit anti-Tetanus Toxin Fragment C Antibody , TTfC, tentoxylysin, tetX

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: This antibody has been tested for use in ELISA and western blot.  For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC.  Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000 - 1:40,000

ELISA Dilution: 1:50,000 - 1:250,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 63 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Tetanus Toxin C-Fragment (TTC)

Background: Background: Tetanus toxin C-fragment (TTc) is a non-toxic 52 kD polypeptide generated by papain cleavage of tetanus toxin, corresponding to the C-terminal 451 amino acids. The illness known as Tetanus is caused by a neurotoxin produced by the anaerobic bacterium Clostridium tetani. C. tetani is commonly found in soil, feces, and on many objects lying on the ground, such as rusty metal, hence puncture wounds are a common cause of tetanus. It acts upon the presynaptic membranes of both central and peripheral nervous systems to block the release of neurotransmitters.   In its toxic form tetanus neurotoxin is a 150 kDa protein, consisting of two major components: a light chain and heavy chain.  The light chain contains the enzymatic portion of the toxin and is responsible for its toxic effects.  The heavy chain binds to the neuron and aids delivery of the light chain to the neuron interior.   A single disulfide bond links light and heavy chains.

Gene ID: 1061100

NCBI Accession: NP_783831

UniProt: P04958