LYZ antibody (AA 1-129)

Catalog No. ABIN964704

Supplier Product No.: 200-401-072

Product Details anti-LYZ Antibody

Target: LYZ (Lysozyme (LYZ))

Binding Specificity: AA 1-129

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Supplier Product No.: 200-401-072

Supplier: Rockland

Purpose: Lysozyme Antibody

Cross-Reactivity (Details): This purified antibody is directed against lysozyme from hen egg white protein.

Characteristics: Synonyms: rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-Lysozyme Hen Egg White purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: LYZ (LYZ Products)

Background: Background: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of β-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers.  Lysozyme is common in animals and plants.  In birds, lysozyme is also an exceptionally abundant protein in egg whites.  Its biological function in fowl eggs is unclear.  Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme.  Lysozyme from domestic goose is designated lysozyme ''g''.  Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis.  Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.

Gene ID: 396218

NCBI Accession: NP_990612

UniProt: P00698