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Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Atto 425) - Preadsorbed

WB, FLISA, FM Polyclonal IgG Atto 425
Catalog No. ABIN964954
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2271
    • 457
    • 363
    • 188
    • 130
    • 123
    • 92
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 684
    • 684
    • 567
    • 436
    • 378
    • 235
    • 199
    • 156
    • 138
    • 130
    • 113
    • 97
    • 95
    • 93
    • 82
    • 58
    • 44
    • 28
    • 27
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1677
    • 1473
    • 546
    • 243
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3419
    • 196
    • 1
    Polyclonal
    Conjugate
    • 578
    • 400
    • 393
    • 350
    • 276
    • 245
    • 191
    • 118
    • 98
    • 66
    • 64
    • 61
    • 58
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 32
    • 32
    • 32
    • 32
    • 31
    • 30
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    Atto 425
    Application
    • 1778
    • 1700
    • 1687
    • 1440
    • 933
    • 909
    • 883
    • 463
    • 243
    • 243
    • 238
    • 100
    • 92
    • 85
    • 76
    • 50
    • 48
    • 32
    • 31
    • 27
    • 24
    • 22
    • 21
    • 16
    • 11
    • 8
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
    Characteristics
    Anti-Mouse IgG (H&L) conjugated by ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.3
  • Application Notes

    Application Note: Anti-Mouse IgG (H&L) conjugated by ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Comment

    The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Goat Anti-Mouse IgG Antibody ATTO425 Conjugation, Goat Anti-Mouse IgG ATTO 425 Conjugated Antibody

    Background: Anti-Mouse IgG ATTO 425 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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