F(ab')2 fragments were generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab')2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab')2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab')2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Anti-Rabbit IgG F(ab')2 Antibody was produced in goat by repeated immunization with Rabbit IgG F(ab')2 fragment. Immunogen Type: Native Protein
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
For Research Use only
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
4 °C/-20 °C
Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.