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Goat anti-Rabbit IgG (F(ab')2 Region) Antibody (HRP) Secondary Antibody

DB, IEM, IHC, ELISA, WB Polyclonal HRP F(ab')2 fragment
Catalog No. ABIN965219
$325.50
Plus shipping costs $45.00
500 μg
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 1529
    • 134
    • 117
    • 46
    • 34
    • 30
    • 24
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reactivity
    • 367
    • 333
    • 314
    • 224
    • 213
    • 111
    • 110
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 821
    • 781
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 12
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 1786
    • 177
    Polyclonal
    Conjugate
    • 317
    • 232
    • 216
    • 207
    • 147
    • 146
    • 128
    • 72
    • 49
    • 46
    • 36
    • 31
    • 29
    • 28
    • 26
    • 26
    • 26
    • 25
    • 25
    • 25
    • 24
    • 24
    • 23
    • 23
    • 23
    • 23
    • 23
    • 22
    • 21
    • 19
    • 19
    • 18
    • 18
    • 14
    • 12
    • 11
    • 10
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Application
    • 1260
    • 1111
    • 916
    • 806
    • 539
    • 525
    • 499
    • 144
    • 100
    • 71
    • 71
    • 65
    • 51
    • 50
    • 29
    • 27
    • 16
    • 13
    • 11
    • 10
    • 10
    • 9
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Specificity
    F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed detects F(ab')2 fragments.
    Characteristics
    F(ab')2 fragments were generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab')2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab')2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab')2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining.
    F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    Immunogen
    Anti-Rabbit IgG F(ab')2 Antibody was produced in goat by repeated immunization with Rabbit IgG F(ab')2 fragment.
    Immunogen Type: Native Protein
  • Application Notes
    Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Preservative
    Gentamicin sulfate
    Handling Advice
    Do NOT add Sodium Azide!
    Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Target
    IgG
    Background
    Synonyms: Rabbit F(ab')2 Antibody, Gt-a-Rabbit F(ab')2 conjugated, Rabbit F(ab')2 Peroxidase Antibody in goat, Rabbit F(ab')2 Peroxidase conjugated F(ab')2 Secondary Antibody.
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