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Goat anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG
Catalog No. ABIN965324
$249.00
Plus shipping costs $45.00
1 mg
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  • Target
    Binding Specificity
    Heavy & Light Chain
    Reactivity
    Guinea Pig
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    FITC
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.
    Characteristics
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Guinea Pig IgG whole molecule

    Isotype
    IgG
    Fragment
    Fab fragment
    Labeling Ratio
    3.26
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
    Expiry Date
    12 months
  • Target
    Background

    Synonyms: Goat Fab Anti-Guinea Pig Antibody Fluorescein Conjugation, Goat Fab Anti-Guinea Pig FITC Conjugated Antibody

    Background: Fab Anti-Guinea Pig IgG Fluorescein Antibody generated in goat detects guinea pig IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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