AMACR antibody

Catalog No. ABIN968957

The Mouse Monoclonal anti-AMACR antibody has been validated for WB, IHC, ELISA and ICC. It is suitable to detect AMACR in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for AMACR antibody (ABIN968957)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 2A10F3

Product Details anti-AMACR Antibody

Purpose: AMACR Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human AMACR expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-AMACR antibody (ABIN968957)

Chen, Watson, Marengo, Decker, Coleman, Nelson, Sikes: "Gene expression in the LNCaP human prostate cancer progression model: progression associated expression in vitro corresponds to expression changes associated with prostate cancer progression in vivo." in: Cancer letters, Vol. 244, Issue 2, pp. 274-88, (2006) (PubMed).

Epstein, Herawi: "Prostate needle biopsies containing prostatic intraepithelial neoplasia or atypical foci suspicious for carcinoma: implications for patient care." in: The Journal of urology, Vol. 175, Issue 3 Pt 1, pp. 820-34, (2006) (PubMed).

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR

Background: AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4), also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Molecular Weight: 42 kDa

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process