CIITA antibody (AA 1-333)

Catalog No. ABIN97339

Supplier Product No.: 100-401-249

Product Details anti-CIITA Antibody

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Binding Specificity: AA 1-333

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIITA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-401-249

Supplier: Rockland

Purpose: CIITA Antibody

Characteristics: Synonyms: rabbit anti-CIITA Antibody, MHC class II transactivator, CIITA, MHC2TA

Purification: Anti-CIITA antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: Anti-CIITA antibody has been tested in western blot. For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 90 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CIITA antibody (ABIN97339)

Majumder, Lee, Rahmberg, Kumar, Mi, Scharer, Boss: "A super enhancer controls expression and chromatin architecture within the MHC class II locus." in: The Journal of experimental medicine, Vol. 217, Issue 2, (2020) (PubMed).

Scharer, Choi, Barwick, Majumder, Lohsen, Boss: "Genome-wide CIITA-binding profile identifies sequence preferences that dictate function versus recruitment." in: Nucleic acids research, Vol. 43, Issue 6, pp. 3128-42, (2015) (PubMed).

Koues, Dudley, Mehta, Greer: "The 19S proteasome positively regulates histone methylation at cytokine inducible genes." in: Biochimica et biophysica acta, Vol. 1789, Issue 11-12, pp. 691-701, (2009) (PubMed).

Target Details for CIITA

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Alternative Name: CIITA (CIITA Products)

Background: Background: Anti-CIITA antibody detects CIITA. The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Gene ID: 4261

NCBI Accession: NP_000237

UniProt: P33076

Pathways: Cancer Immune Checkpoints