Glucose Oxidase antibody

Catalog No. ABIN99707

Supplier Product No.: 100-4137

Product Details anti-Glucose Oxidase Antibody

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 100-4137

Supplier: Rockland

Purpose: Glucose Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glucose Oxidase [Aspergillus niger].

Characteristics: Synonyms: rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Anti-Glucose Oxidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Glucose Oxidase Antibody has been assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Rabbit) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for Anti-Glucose Oxidase Antibody.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase (GOD Products)

Background: Background: Glucose Oxidase, first described in Aspergillus niger), catalyzes the oxidation of glucose into gluconic acid whilst in an aerobic environment. Glucose Oxidase is one of the most widely used biomarkers due to the large enthalpy change observed when it oxidizes glucose. Glucose oxidase can be used as a label in immunoassays when attached to antibodies and for staining tissues. In respect to glucose oxidases' ability to stain tissues, it is most commonly used to determine the levels of glucose in blood, serum, and plasma, as part of colorimetric assays. Anti-Glucose Oxidase Antibody is ideal for investigators in Immunology, Molecular Biology, and Hematology Research.

UniProt: P13006