GST antibody (HRP)

Catalog No. ABIN99866

Supplier Product No.: 600-103-200

Product Details anti-GST Antibody (HRP)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-103-200

Supplier: Rockland

Purpose: GST Antibody HRP Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Characteristics: Synonyms: goat anti-GST antibody HRP conjugation, peroxidase conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody

Purification: Anti-GST HRP Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-GST peroxidase conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using peroxidase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:5,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store anti-GST at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GST antibody (HRP) (ABIN99866)

Lau, McAdam, Bergseth, Grevys, Bruun, Ludviksen, Fure, Espevik, Moen, Andersen, Mollnes: "NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies." in: mAbs, Vol. 12, Issue 1, pp. 1686319, (2020) (PubMed).

Lee, Romain, Yan, Watanabe, Charab, Todorova, Lee, Triplett, Donkor, Lungu, Lux, Marshall, Lindorfer, Goff, Balbino, Kang, Tanno, Delidakis, Alford, Taylor, Nimmerjahn, Varadarajan, Bruhns, Zhang et al.: "IgG Fc domains that bind C1q but not effector Fcγ receptors delineate the importance of complement-mediated effector functions. ..." in: Nature immunology, Vol. 18, Issue 8, pp. 889-898, (2017) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Background: Background: GST Antibody HRP Conjugated is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag. Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.