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GST antibody (HRP)

GST Reactivity: Schistosoma japonicum WB, ELISA, IHC Host: Goat Polyclonal HRP
Rockland
Catalog No. ABIN99866
Supplier Product No.: 600-103-200
  • Target See all GST Antibodies
    GST (Glutathione S Transferase (GST))
    Reactivity
    • 95
    • 29
    • 14
    • 9
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Schistosoma japonicum
    Host
    • 60
    • 54
    • 46
    • 2
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 99
    • 64
    Polyclonal
    Conjugate
    • 74
    • 11
    • 10
    • 10
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GST antibody is conjugated to HRP
    Application
    • 147
    • 77
    • 37
    • 30
    • 26
    • 25
    • 15
    • 11
    • 9
    • 9
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-103-200
    Supplier
    Rockland
    Purpose
    GST Antibody HRP Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
    Characteristics
    Synonyms: goat anti-GST antibody HRP conjugation, peroxidase conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody
    Purification
    Anti-GST HRP Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product GST Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Anti-GST peroxidase conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using peroxidase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

    Western Blot Dilution: 1:2,000 - 1:5,000

    ELISA Dilution: 1:20,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store anti-GST at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Lau, McAdam, Bergseth, Grevys, Bruun, Ludviksen, Fure, Espevik, Moen, Andersen, Mollnes: "NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies." in: mAbs, Vol. 12, Issue 1, pp. 1686319, (2020) (PubMed).

    Lee, Romain, Yan, Watanabe, Charab, Todorova, Lee, Triplett, Donkor, Lungu, Lux, Marshall, Lindorfer, Goff, Balbino, Kang, Tanno, Delidakis, Alford, Taylor, Nimmerjahn, Varadarajan, Bruhns, Zhang et al.: "IgG Fc domains that bind C1q but not effector Fcγ receptors delineate the importance of complement-mediated effector functions. ..." in: Nature immunology, Vol. 18, Issue 8, pp. 889-898, (2017) (PubMed).

  • Target
    GST (Glutathione S Transferase (GST))
    Alternative Name
    GST (GST Products)
    Background
    Background: GST Antibody HRP Conjugated is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag. Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
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